For the algal oil extraction experiments from wet algal biomass,
a given amount of wet algal biomass (equal to 0.3 g dry biomass)
was extracted with various solvents in a conical flask with magnetic
stirring (400 rpm, 5 min) at room temperature. The crude extracts
were collected and evaporated in a rotary evaporator to get
the residual crude oil. The resulted crude algal oil was further purified
to obtain the algal oil according to the protocol mentioned
above. The resulted algal oil from the wet algal biomass was
weighed as W2.