The present investigations were undertaken to evaluate the F2 intergeneric population of Carica papaya (var. Pusa Nanha, CP 50 and CO 7) and Vasconcellea cauliflora for PRSV resistance. Investigations were also made to estimate the mean performance, genetic parameters governing different traits and reaction to the PRSV in the F2 population. Among the crosses, Pusa Nanha × V. cauliflora recorded superior mean performance for fruit yield, number of fruits per tree and fruit biochemical parameters than other two crosses in F2 generation. Mean performance for papain recovery per fruit and the enzyme activity were high in CP 50 × V. cauliflora. Fruit quality parameters were high in CO 7 × V. cauliflora. Desirable mean per- formance for days taken for disease after inoculation and disease intensity score were recorded by Pusa Nanha × V. cauliflora. Among the various characters studied, higher GCV, PCV, heritability and genetic advance as percent of mean were registered by the cross Pusa Nanha × V. cauliflora for all the morpholog- ical characters and fruit yield parameters. From the F2 population 24 plus trees were selected for further evaluation based on their performance viz., morphological, yield, quality and biochemical characters and reaction to PRSV.