The composition of adsorbed proteins at the oil droplet surface after 24-h storage was determined by SDS–PAGE with 2-mercaptoethanol (Fig. 6). Since the presence of 2-Me scarcely affected the protein profile, the profile with 2-mercaptoethanol was only shown here (Fig. 6). In the profile of control emulsions, many kinds of protein bands diffusing in the wide range of molecular weight were observed in addition to the main components such as α-, β-, κ-casein, β-lactoglobulin and α-lactalbumin. The total intensity of stained bands in the control profile was higher than that in the emulsion with compositions 1–3. It was shown, in the previous section, that the amount of adsorbed proteins ranked in the following order; control > composition 1 > composition 2 > composition 3. Since the same amount of cream was treated for the SDS–PAGE experiment as described in “Section 2”, it is natural that the total intensity of each emulsion sample ranked in the same order. High-mass materials were found at the top of the gel of all the emulsion samples, suggesting the protein aggregation induced by the severe heat treatment for sterilization.