used on bulk tissues which still retained antigenic
determinants. Moreover, the electron microscope
localization of specific reaction product on individual
VLDL particles suggests an increased sensitivity
for localizing small amounts of intracellularprotein since nascent VLDL are only about 400/~
in diameter and contain only 5-7% protein by
weight (22). These improvements probably result
from the method of fixation by perfusion through
the hepatic vascular bed. Perfusion of only 0.25%