Hot Cayenne pepper (Capsicum frutescens) was used for the development of the MAE method. The peppers were peeled, and the stalk and seeds were separated from the rest. Only the pericarp and the placenta of the pepper have been used for this study. Both the pericarp and the placenta were triturated with a conventional electric mixer, until a homogeneous sample was obtained for the analysis. The triturated sample obtained was preserved in the freezer at −20 ◦C until analysed