To speed up research on the usefulness and selection of bacterial starter cultures for cocoa bean
fermentation, a benchmark cocoa bean fermentation process under natural fermentation conditions was
developed successfully. Therefore, spontaneous fermentations of cocoa pulp-bean mass in vessels on a 20
kg scale were tried out in triplicate. The community dynamics and kinetics of these fermentations were
studied through a multiphasic approach. Microbiological analysis revealed a limited bacterial species
diversity and targeted community dynamics of both lactic acid bacteria (LAB) and acetic acid bacteria
(AAB) during fermentation, as was the case during cocoa bean fermentations processes carried out in the
field. LAB isolates belonged to two main (GTG)5-PCR clusters, namely Lactobacillus plantarum and
Lactobacillus fermentum, with Fructobacillus pseudofilculneus occurring occasionally; one main (GTG)5-
PCR cluster, composed of Acetobacter pasteurianus, was found among the AAB isolates, besides minor
clusters of Acetobacter ghanensis and Acetobacter senegalensis. 16S rRNA-PCR-DGGE revealed that L.
plantarum and L. fermentum dominated the fermentations from day two until the end and Acetobacter
was the only AAB species present at the end of the fermentations. Also, species of Tatumella and Pantoea
were detected culture-independently at the beginning of the fermentations. Further, it was shown
through metabolite target analyses that similar substrate consumption and metabolite production
kinetics occurred in the vessels compared to spontaneous cocoa bean fermentation processes. Current
drawbacks of the vessel fermentations encompassed an insufficient mixing of the cocoa pulp-bean mass
and retarded yeast growth.
To speed up research on the usefulness and selection of bacterial starter cultures for cocoa beanfermentation, a benchmark cocoa bean fermentation process under natural fermentation conditions wasdeveloped successfully. Therefore, spontaneous fermentations of cocoa pulp-bean mass in vessels on a 20kg scale were tried out in triplicate. The community dynamics and kinetics of these fermentations werestudied through a multiphasic approach. Microbiological analysis revealed a limited bacterial speciesdiversity and targeted community dynamics of both lactic acid bacteria (LAB) and acetic acid bacteria(AAB) during fermentation, as was the case during cocoa bean fermentations processes carried out in thefield. LAB isolates belonged to two main (GTG)5-PCR clusters, namely Lactobacillus plantarum andLactobacillus fermentum, with Fructobacillus pseudofilculneus occurring occasionally; one main (GTG)5-PCR cluster, composed of Acetobacter pasteurianus, was found among the AAB isolates, besides minorclusters of Acetobacter ghanensis and Acetobacter senegalensis. 16S rRNA-PCR-DGGE revealed that L.plantarum and L. fermentum dominated the fermentations from day two until the end and Acetobacterwas the only AAB species present at the end of the fermentations. Also, species of Tatumella and Pantoeawere detected culture-independently at the beginning of the fermentations. Further, it was shownthrough metabolite target analyses that similar substrate consumption and metabolite productionจลนพลศาสตร์เกิดในเรือเปรียบเทียบกับกระบวนการหมักถั่วโกโก้อยู่ ปัจจุบันข้อเสียของการหมักแหนมที่เรือผ่านเป็นพอผสมของโกโก้เยื่อถั่วจำนวนมากและปัญญาอ่อนยีสต์เจริญเติบโต
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To speed up research on the usefulness and selection of bacterial starter cultures for cocoa bean
fermentation, a benchmark cocoa bean fermentation process under natural fermentation conditions was
developed successfully. Therefore, spontaneous fermentations of cocoa pulp-bean mass in vessels on a 20
kg scale were tried out in triplicate. The community dynamics and kinetics of these fermentations were
studied through a multiphasic approach. Microbiological analysis revealed a limited bacterial species
diversity and targeted community dynamics of both lactic acid bacteria (LAB) and acetic acid bacteria
(AAB) during fermentation, as was the case during cocoa bean fermentations processes carried out in the
field. LAB isolates belonged to two main (GTG)5-PCR clusters, namely Lactobacillus plantarum and
Lactobacillus fermentum, with Fructobacillus pseudofilculneus occurring occasionally; one main (GTG)5-
PCR cluster, composed of Acetobacter pasteurianus, was found among the AAB isolates, besides minor
clusters of Acetobacter ghanensis and Acetobacter senegalensis. 16S rRNA-PCR-DGGE revealed that L.
plantarum and L. fermentum dominated the fermentations from day two until the end and Acetobacter
was the only AAB species present at the end of the fermentations. Also, species of Tatumella and Pantoea
were detected culture-independently at the beginning of the fermentations. Further, it was shown
through metabolite target analyses that similar substrate consumption and metabolite production
kinetics occurred in the vessels compared to spontaneous cocoa bean fermentation processes. Current
drawbacks of the vessel fermentations encompassed an insufficient mixing of the cocoa pulp-bean mass
and retarded yeast growth.
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