2.7. Antioxidant activities
Antiradical activity (AA) was determined using an improved
ABTS decolorization assay (Re et al., 1999). Chelating power (CHEL)
was determined by the method of Guo, Lee, Chiang, Lin, and Chang
(2001). The degree of inhibition of linoleic acid peroxidation (LPO)
was performed according to Kuo, Yeh and Pan (1999). All activities
were expressed as EC50 e extract concentration provided 50% of
activity based on dose-dependent mode of action. Reducing power
(RP) was determined using the method described by Oyaizu (1986).
EC50 value (mg/mL) is the effective concentration at which the
absorbance was 0.5 for reducing power and was obtained by
interpolation from linear regression analysis.