Expression of the bHLH Lc transgene in Lc petunia was
shown to be insensitive to the light treatment (Fig. 6A), and
therefore not responsible for the light-induced phenotype. A
WD40 factor (An11) is required for floral anthocyanin
regulation in petunia in addition to MYB and bHLH
factors. Semi-quantitative RT-PCR showed similar transcript
levels for An11 in both Mitchell and Lc petunia
leaves, in either shade or high-light conditions (Fig. 6B).