follicles using an 18-gauge needle attached to a 10-mL syringe. The follicular aspirations for Nelore and B. t. taurus
cows was conducted simultaneously, i.e., 6–7 h after collection of the ovaries. Therefore, for Nelore breed the ovaries stayed in the laboratory (in the thermocontainer) for 4–5 h before follicle aspiration(Experiment 2). The oocytes were classified according to their morphology