Table 4 shows the diacetyl produced by the isolates, it ranged within 0.91-1.92 (L. plantarum LPF2). The highest diacetyl was produced at 36 hrs of incubation. Reasonable quantity of dicaetyl was produced by the screened isolates. Diacetyl has a strong, buttery flavor and is essential at low concentrations in many dairy products, such as butter, buttermilk and fresh cheese.