Shoot Induction in Nodal and Shoot Tip Explants
It is inferred from Table 1 that the multi nodal explants with different combinations ceand concentration of auxins and cytokinins responded better to survival and bud emergen than single node explants. Ninety percent survival and bud emergence was reported under multi nodal (stem and shoot tip) when inoculated in basal medium along with different combinations and concentrations of growth regulators. While, in case of basal medium alone the survival of multi nodal explants was found to be only 25%. Less than 40% survival was observed when single node explants (stem and shoot tip) were used and cultured in different combinations of basal medium and PGR, while it was less than 15% when cultured in basal medium alone. The enrichment of the medium with various combinations of cytokinins (BAP & KN) and auxins (NAA & IAA) did not contribute to multiple shoot formation (Table 1).
The shoots originated from the resident meristem of the nodal explants (single or multiple) of N. khasiana later grew into small branches with green leaves. The newly formed shoots achieved an average growth of 3-4 cm in 70 days, whereas in the basal medium alone the average shoot length was only 1.0-1.5 cm in 105 days of inoculation in the sub cultures (Table 2).