The mycelium was then scraped from the surface of the plate and crushed with a mortar in 1 mL of lysis solution (2% Triton-X 100, 1% SDS, 100 mM NaCl, 10 mM Tris-HCl, pH 8.0) and 10 µL of protease K (10 mg/mL).
The myceliumwas then scraped from the surface of the plate and crushedwith a mortar in 1 mL of lysis solution (2% Triton-X 100, 1%SDS, 100 mM NaCl, 10 mM Tris-HCl, pH 8.0) and 10 µLof protease K (10 mg/mL).