The purpose of the study des was to investigate and associate biology teacher international behaviours in 8 scales of the actual and preferred of the 48-Item Questionnaire on Teacher Interaction (QTI) with a sample of 134 biology upper secondary educational students at Grade 11 in Sarakham Pittayakom School in Mahasarakham. Relationships between actual students’ perceptions of their teacher interpersonal behaviours toward their Test Of Biology-Related Attitude (TOBRA) to their biology laboratory classes were associated with the Test of Science-Related Attitude (TOSRA). The reliability of the QTI scales ranged from 0.60 to 0.83 of the actual from and ranged from 0.71 to 0.87. The preferred teachers could be identified as those whose students' perceptions were more than one standard deviation above the mean on the scales of Leadership, Helping/Friendly, Understanding, and Student Responsibility and Freedom and less than one standard deviation below the mean on the Uncertainty, Dissatisfied, Admonishing, and Strict scales. The QTI may thus provide a basis for systematic attempts to improve one's own teaching practice. Statistically significant differences were found between the students’ perceptions of actual and preferred teacher interpersonal behaviours of their biology classes also were found, while confirmatory factor analyses provided support for the theoretical framework behind the questionnaire (0.41-0.93 omitted). The multiple correlations R2 was significant for the QTI and considered associations with the TOBRA, and value indicates that 44% of the variance in students’ attitude of actual chemistry classed was also determined.