Protocol
1.
Cool the required volume of acetone to -20
°
C.
2.
Place protein sample in acetone-compatible tube.
3.
Add four times the sample volume of
cold (-20°C) acetone to the tube.
4.
Vortex tube and incubate for 60 minutes at -20°C.
5.
Centrifuge 10 minutes at 13,000-15,000 ×
g
.
6.
Decant and properly dispose of the supernatant, be
ing careful to not dislodge the protein pellet.
Optional:
If additional cycles of precipitation
are necessary to completely remove th
e interfering substance, then repeat
steps 2-5 before proceeding to step 7.
7.
Allow the acetone to evaporate from the uncapped tube at room
temperature for 30 minutes. Do not over-dry pellet, or it
may not dissolve properly.
8.
Add buffer appropriate for the downstream process and vortex thoroughly to dissolve protein pellet.