The explants were sterilized and cultured on MS medium containing different concentrations of
growth regulators naphthalene acetic acid (NAA) or indole-3-butyric acid (IBA) with 0.5 mg/L of
kinetin (Kin) callus formation was 70.2% after 45 days of incubation in dark on medium supplemented
with 1.5 mg/L of NAA. After one month of callus culture on medium supplemented with
2 mg/L BA the shoot number was 5.12 and for the multiplication stage. The shoot number was
4.21 and length was 6.17 cm on medium supplemented with 1 mg/L Kin +0.1 mg/L NAA.
DPPH reagent was used to test the antioxidant activity. The aqueous and methanol extracts of
in vitro plants which were treated with 1.5 and 1 mg/L of kin plus 0.1 mg/L of NAA showed a strong
DPPH scavenging activity where IC50 was 0.307 and 0.369 mg/ml, respectively, while the IC50 of aqueous
and methanol extracts of wild plants was 0.516 and 9.229 mg/ml, respectively. Our results suggested
that plant growth regulators and in vitro culture conditions increased the antioxidant activity