Artificial contamination
15 pasteurized milk samples and 15 UHT milk samples randomly selected were artificially contaminated. These samples were divided into two groups, one group was contaminated with a culture of the Enterobacteriaceae family (Salmonella Typhimurium ATCC 14028) and another group with a negative control culture, a strain of Staphylococcus aureus (ATCC 02923). The cultures were reactivated in nutrient broth (Oxoid, Ltd, Basingstoke, UK) and incubated at 37 °C for 18 h (Owen et al., 2010).
Artificial contamination15 pasteurized milk samples and 15 UHT milk samples randomly selected were artificially contaminated. These samples were divided into two groups, one group was contaminated with a culture of the Enterobacteriaceae family (Salmonella Typhimurium ATCC 14028) and another group with a negative control culture, a strain of Staphylococcus aureus (ATCC 02923). The cultures were reactivated in nutrient broth (Oxoid, Ltd, Basingstoke, UK) and incubated at 37 °C for 18 h (Owen et al., 2010).
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