PCR can detect coliform bacteria using lacZ gene (gene β-galacotozidase) and E. coli bacteria using uidA gene
(β-glucuronidase gene)[12]. In the present study, common ways
to identify coliforms are compared with the new molecular
methods and the sensitivity and precision of these methods is
evaluated in practical application. In this study, different items
such as molecular method for reducing the volume of routine
operations in the Iran’s water bacteriology laboratory, reduction
in the costs of samples analysis, increase in the accuracy and
possible coverage of VBNC bacteria in the water by molecular
methods are presented. Plan targeting is based on PCR method
in the initial screening of all samples accepted per day. As high
percentage of sample accepted each day have negative test
results, initial screening by PCR, removal of samples having
negative test results and focus of tests on positive samples cause
reduction in the consumption of a high volume of medium
and involvement of the expert in the creation and removal of
media.