In a preliminary
laboratory study, a 15-ml sample of wine (pH 3.55; ethanol,
12%; free SO2, 20 mg/liter) was incubated in a 20-ml test tube
at 19°C. During the course of 8 days, counts, consisting of a
mixed population of A. aceti and A. pasteurianus, progressively
increased from an initial level of 4 x 102 cells per ml to
1.1 x 105 cells per ml.