The three isolates under study were identified by MALDI-TOF MS as ‘Fusarium species’ by an in-house database for Fusarium species with confidence log scores 2.193, 2.20, and 2.226, respectively indicating correct generic and probable specific identification. The strains CBS 125177, CBS 125178, and CBS 125181 had initially been identified morphologically as Fusariumandiyazi. Two separate clusters were visualized by using MALDI-TOF MS, one comprising three strains including the type strain of F. andiyazi, and another with strains CBS 125177, CBS 125178, and CBS 125181 ( Fig 5). This indicates that MALDI-TOF MS has a potential to distinguish closely related and morphologically identical Fusarium species. Here, the MALDI-TOF MS data are used as an additional parameter in our polyphasic classification of this cryptic species.