PHA accumulation. They are however known to control the
amount [106,108], size and number of granules [111], and
possibly also help prevent PHA crystallization [112]. In the
absence of phasins, cells accumulate only one very large
granule, taking up all available space in the cell [111]. It has
also been shown that overexpression of the phasin leads to
smaller granules [99]. Phasins can bind to any hydrophobic
inclusion regardless of its composition [3] and appear
to bind to the synthase when conditions are not right for
PHA accumulation. Cells have been found to generally stop
increasing their P(3HB) content at around 80–90% of the
cell dry mass. It is known that PHA synthase activity and
intracellular monomer concentration remain high at this
point, so it is likely that physical constraints are at least
one limiting factor for polymer accumulation [113].
PHA accumulation. They are however known to control theamount [106,108], size and number of granules [111], andpossibly also help prevent PHA crystallization [112]. In theabsence of phasins, cells accumulate only one very largegranule, taking up all available space in the cell [111]. It hasalso been shown that overexpression of the phasin leads tosmaller granules [99]. Phasins can bind to any hydrophobicinclusion regardless of its composition [3] and appearto bind to the synthase when conditions are not right forPHA accumulation. Cells have been found to generally stopincreasing their P(3HB) content at around 80–90% of thecell dry mass. It is known that PHA synthase activity andintracellular monomer concentration remain high at thispoint, so it is likely that physical constraints are at leastone limiting factor for polymer accumulation [113].
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