The P450-Glo™ Assays provide a luminogenic CYP substrate, a lyophilized Luciferin Detection Reagent and a
reconstitution buffer. The user supplies a CYP preparation with the requisite buffer and NADPH, which is supplied by
the NADPH Regeneration System (Cat.# V9510). In cell-based assays, NADPH in the cell is sufficient to support CYP
activity. Protocols are configured for multiwell plate formats but can be easily adapted for single-tube applications. An
overview of the protocol is provided in Figure 2.