Figure 6. Histopathology of liver samples after treatment with different concentrations of
volatile oil from Blumea balsamifera (BB oil) wound bearing rats. (A) Group B1: Control
(the blank group) showed normal liver structure. (B) Group B2: Olive oil (vehicle) showed
diffused vacuolar changes. (C) Group B3: BB oil (100% w/v) 1000 mg/kg showed diffused
vacuolar changes; hepatocytes appeared pale with small discrete cytoplasmic vacuoles
(arrow), displaying clear cytoplasm and pyknotic nuclei (circles). These results suggested
an early mild hepatocellular necrosis; however, the lesions were reduced compared with
A3 (normal group). (D) Group B4: BB oil (50% w/v) 1000 mg/kg showed diffused
vacuolar changes and appeared pale with small discrete cytoplasmic vacuoles (arrow).
Moreover, some hepatocyte pyknotic nuclei were observed (circles). (E) Group B5: BB oil
(20% w/v) 1000 mg/kg showed diffused vacuolar changes; hepatocytes appeared mild
cytoplasmic vacuoles (arrow) and slight amounts of pyknotic nuclei (circles).