M. alba var. ‘Shidareguwa’ is perennial plant shrub species. There are several types of leaves utilized to feed the silkworms (Bombyxmori L.) Some are planted as ornamental and landscape plants because of their graceful, intriguing forms and tolerate pruning. and use female “Shidareguwa” mulberry (dioecious) for this experiment, and is known for being difficult to propagate by hardwood cuttings and a seedless (Yamanouchi et al, 2009) Therefore, the application of biotechnology is to help the propagation which leaves explant culture on the MS medium supplemented with various combination of growth regulators as follows : 2,4-dichlorophenoxyacetic acid (2,4-D) and Bencylaminopurine (BA), and 6-(Y,Y-Dimethylallylamino)purine (2iP)