The biomass represented as dry cell weight (DCW e (g/L)) was
determined by the gravimetric method. The total carbohydrate
content of the samples (cell-free supernatant) was determined
according to the phenol sulphuric acid method described by Dubois
et al. [13]. The amount of carbohydrates was determined by using
Varian Cary Bio 100 UVeVisible spectrophotometer at 490 nm
against the blank. The total reducing sugar amountwas determined
according to the assay given by Somogyi [14]. The absorbance was
read on Varian Cary Bio 100 UVeVisible spectrophotometer at
500 nm against water.