Pseudomonas veronii 2E, Delftia acidovorans AR, Klebsiella oxytoca P2 and Klebsiella ornithinolytica 1P, isolated from polluted environments showed a decrease from 38.83 to 74.32%, in 0.05 mM of initial Cr(VI). As revealed DGGE experiments, P. veronii 2E and K. ornithinolytica 1P could develop together in cocultures and in these conditions a 72.88% of Cr(VI) present wasremoved.