The supernatant was collected and used for total protein estimation by Bradford assay. SDS–PAGE analysis was also carried out to determine qualitatively the effect of ethanol on the protein expression profile on 15% (w/v) Bis–Tris gel.
The supernatant was collected andused for total protein estimation by Bradford assay. SDS–PAGEanalysis was also carried out to determine qualitatively the effectof ethanol on the protein expression profile on 15% (w/v) Bis–Trisgel.