Incubation was performed on a reciprocal shaker, at 150 rpm in the dark at 25 ± 2 C. After 2 week of incubation, the cultures were centrifuged at 11,000 rpm for 15 min to harvest the supernatant.
Incubation was performed on a reciprocal shaker, at 150 rpm in the dark at 25 ± 2 C. After 2 week of incubation, thecultures were centrifuged at 11,000 rpm for 15 min to harvest the supernatant.