Vinegar fermentation, which has a long history starting from ca. 3000 BC, is a two-step process: First, the anaerobic conversion of sugars to ethanol and then the aerobic oxidation of ethanol to acetic acid. In the present study, we aimed at producing vinegar with an H. erinaceus fermentation. The quality characteristics of H. erinaceus vinegar were monitored during fermentation by measuring pH, °Brix, color value and microbiological analyses. Of particular interest was the ability to retain antioxidant properties throughout the fermentation process. Total phenolic content and antioxidant (DPPH, ABTS) properties of H. erinaceus vinegar were also profiled.