Fourteen yeast strains that had not previously been characterized
for organic acid production, including eight from the
genus Pseudozyma, were screened for organic acid biosynthesis
using the nitrogen-limited screening medium described (C/N
ratio = 352) (Table 1). Most of the strains tested did not grow well
or did not produce significant amounts of acids when grown on
glycerol. However, when the strains were grown on glucose,