Fig. 3. The inhibitory effects of polysaccharides from brown algae A. marginata andA. angusta on colony formation on human colon cancer cells HT-29, human breastcancer T-47D and human skin melanoma SK-MEL-28 cell lines comparing untreatedcontrol cells. Cells (2.4 × 104/mL) treated with/without polysaccharides (100 g/mL)were exposed in 1 mL of 0.3% BME’s agar containing 10% FBS. The culture was main-tained at 37◦C in a 5% CO2atmosphere for 2 weeks. The colonies were counted undera microscope with the aid of the ImageJ soft ware program. Data are representedas the means SD of the number of colonies are determined from three independentexperiments.