iii) Scanning electron microscopical (SEM) study
For scanning electron microscopical (SEM) study, the
thymus gland of A. panchax specimens were fixed in 2.5%
glutaraldehyde in 0.1 (M) phosphate buffer (pH. 7.2 – 7.4)
at 4° C for 1 –2 hours. After completion of the fixation,
the samples were then rinsed in same buffer i.e., 0.1 (M)
phosphate buffer (pH 7.2 – 7.4) for 3 changes at a regular
interval of 15 minutes and dehydrated in graded chilled
acetone.