Detection of pathogenic vibrio species was done by using 25 g of sample on pre-enrichment, ASPW (Alkaline saline peptone water) spread on TCBS (Thiosulfate citrate bile salts sucrose) (Liofilchem) and TSAT (Soya peptone triphenyl chloride agar) (ISO, 2007).
Detection of non-pathogenic vibrio colonies was performed according to