The Hibiscus genus (Malvaceae) is one of the most popular ornamental plants cultivated in tropical and subtropical regions because it presents several species with various attractive flower colors. Species of the genus Hibiscus, such as Hibiscus rosasinensis,are particularly appreciated for the color of the flowers,with the base of the corolla forming a deep-colored heart and a bright red limb. The main compounds responsible for the characteristic heterogeneous color pattern of Hibiscus flowers in red cultivars are anthocyanins, being preferentially accumulated on calyces. More specifically, the major anthocyanins described in Hibiscus spp. are delphinidin and cyanidin derivatives glycosylated with various sugar moieties and occasionally acylated with organic acids and other phenolic compounds also present in the flower organs.