Aphid acceptance was tested in a no-choice settling test (Banet al., 2008). Ten mixed-instar apterous aphids were placed inside the cylinder as described in Section 5.4 and the number of aphids
settled (i.e. not walking) on the leaf was counted after 2 h. This is
sufficient time for aphids to settle and reach the phloem (Prado
and Tjallingii, 1997). The tests were done under the same conditions
as for aphid infestations but in a separate growth chamber.