Thailand is home to a variety of fermented food products in every region. However, little information is available on the characterisation of bacteriocin-producing Weissella species. Interestingly, only 3 species of Weissella have been reported to produce bacteriocin (Weissella cibaria, Weissella confusa and Weissella paramesenteroides). The objective of this study was to isolate and characterise a new bacteriocin produced by W. cibaria N23, which was isolated from Thai fermented meat and fish products.