Based on the description above, cellulase has an important role in the bioconversion of cellulose to glucose as material for
ethanol production, so it requires optimization for the production of raw cellulases from fungus Trichoderma reeesei. Treatment was conducted by fermentation process for 5 d, 7 d, and 9 d, at temperature 27 °C and 37 °C which is expected to generate an optimal raw cellulase enzyme that can be used to convert the lignocelluloses materials to glucose. This study was conducted to determine the optimum combination of fermentation time and temperature for producing raw cellulase enzymes with high activity of fungus Trichoderma reesei.