resulting in increased 2-oxoglutarate formation. GDH activity (23.7 103 U) in
B was more than 8-fold higher than that (2.78 103 U) in A. Thus
the increase of GDH was greater than that of ODHC, and there was
enhanced flux from 2-oxoglutarate to glutamate but not to succinyl-CoA, resulting in intracellular accumulation of glutamate and
increase in yield of c-PGA. Wu et al. (2008) reported that both increased ICDH activity and decreased ODHC activity led to enhanced
c-PGA production in B. subtilis CGMCC 0833.