Characterization of regenerants
Ploidy level of acclimatized plants was determined by flow
cytometry according to Kielkowska and Adamus (2010).
Potted plants were grown in the greenhouse for about
4 months and then they were vernalized for the next
3 months at 10 ± 2C to induce flowering. Pollen viability
was evaluated according to the Alexander’s procedure
(1969) based on differential staining of aborted (colored as
light green) and non-aborted grains (colored as magentared)
(Fig. 1p). When the stigma was receptive the flowers
were self-pollinated and seed set was scored.