The saline spore suspension of C. militaris 3936 was prepared and inoculated on PDA petri-plates followed by incubation at 20°C for 7 days. The inoculum was prepared by punching out 5mm of PDA discs with sterilized cork borer. The discs containing cultures of C. militaris 3936 were inoculated into 250 ml Erlenmeyer flasks with 100 ml of basal medium (glucose 1.5 %, peptone 0.5 %, KH2PO4 0.3 %, K2HPO4 0.1 %, MgSO4 0.05 %, NaCl 0.05 %) at 25°C on a rotary shaker incubator (110 rpm) for 3 days.