DISCUSSION
Cylindroid, solitary, with mucron, late syzygy and coelomic
habitat of the parasite in an earthworm justify the inclusion of
the present form under the family Monocystidae, subfamily
Monocystinae and genus Monocystis Stein, 1848. Of more
than 70 species belonging to the genus Monocystis Stein,
1848, only 13 have been described from India. The present
monocystid gregarine obtained from the seminal vesicles of
the earthworm Amynthas hawayanus has been compared with
other species of the genus Monocystis and found that it
resembles only two Indian species, Monocystis clubae
Bandyopadhyay et al. 2006a and M. metaphirae
Bandyopadhyay et al. 2006b. The species under discussion
resembles M. clubae in having anterior broad posterior narrow
end only. But the measurements are significantly different.
The new species under discussion is smaller in size (66 x 77
versus 160 x 64 in M. clubae). The anterior end of M. clubae
is rounded while that of the new species contains a cup-like
depression at the anterior end. The anterior end of the gamont
of M. clubae gradually tapers to a narrow tail, but the gamont
of the new species does not end to any tail-like structure. In M.
clubae the nucleus is elongated, that is oval in the proposed
new species. The gametocyst of M. clubae with two unequal
gametocytes, but in the new species the gametocyst with two
almost equal gametocytes. The new species also smaller than
M. metaphirae ( 66 x 77 versus 119x 66 in M. metaphirae).
The gamont of M. metaphirae gradually tapers to rounded
posterior end without any constriction in between the two
ends. But in the new species obtained from the Amynthas
hawayanus, a constriction is present between the two ends.
The anterior tip of the anterior end of the new species contains
a cup-like depression, which is rounded and without any such
depression in the M. metaphirae. The main differences
between the two species is the presence of syzygy in the life
cycle of M. metaphirae, which is completely absent in the new
species. Oocysts shape is also different in both the species.
DISCUSSION
Cylindroid, solitary, with mucron, late syzygy and coelomic
habitat of the parasite in an earthworm justify the inclusion of
the present form under the family Monocystidae, subfamily
Monocystinae and genus Monocystis Stein, 1848. Of more
than 70 species belonging to the genus Monocystis Stein,
1848, only 13 have been described from India. The present
monocystid gregarine obtained from the seminal vesicles of
the earthworm Amynthas hawayanus has been compared with
other species of the genus Monocystis and found that it
resembles only two Indian species, Monocystis clubae
Bandyopadhyay et al. 2006a and M. metaphirae
Bandyopadhyay et al. 2006b. The species under discussion
resembles M. clubae in having anterior broad posterior narrow
end only. But the measurements are significantly different.
The new species under discussion is smaller in size (66 x 77
versus 160 x 64 in M. clubae). The anterior end of M. clubae
is rounded while that of the new species contains a cup-like
depression at the anterior end. The anterior end of the gamont
of M. clubae gradually tapers to a narrow tail, but the gamont
of the new species does not end to any tail-like structure. In M.
clubae the nucleus is elongated, that is oval in the proposed
new species. The gametocyst of M. clubae with two unequal
gametocytes, but in the new species the gametocyst with two
almost equal gametocytes. The new species also smaller than
M. metaphirae ( 66 x 77 versus 119x 66 in M. metaphirae).
The gamont of M. metaphirae gradually tapers to rounded
posterior end without any constriction in between the two
ends. But in the new species obtained from the Amynthas
hawayanus, a constriction is present between the two ends.
The anterior tip of the anterior end of the new species contains
a cup-like depression, which is rounded and without any such
depression in the M. metaphirae. The main differences
between the two species is the presence of syzygy in the life
cycle of M. metaphirae, which is completely absent in the new
species. Oocysts shape is also different in both the species.
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