Properties such as surface morphology and wettability influence the ability of cells to adhere to a particular surface. The results of this study demonstrate that the incorporation of essential oil compo- nents does not cause significant changes of the surface homoge- neity while making the EVA film surface less hydrophobic. This effect could play an important role in the initial phase of the bacterial adhesion because a reduced hydrophobicity causes a weaker attractive strength and a lower interaction between the surface and the bacteria, thus compromising the following phase of biomass formation.