2.6. Growth of shoots and microtubers
After three weeks, shoot growth was studied by measuring (1)
number of shoots, (2) length of shoots and (3) number of internodes. The total chlorophyll content of fully developed leaves was
measured by a UV–vis spectrophotometer at 645 and 663 nm following the method ofHiscox and Israelstam (1979)and results are
presented inTable 2. Occurrence of hyperhydricity was detected
by visible symptoms including thick, broad, translucent leaves that
were wrinkled or curled, or both, and brittle (Table 3). Data on
microtuberization was recorded after 10 weeks of culture; the
number and fresh weight of microtubers was measured as an index
of their quality
2.6. Growth of shoots and microtubersAfter three weeks, shoot growth was studied by measuring (1)number of shoots, (2) length of shoots and (3) number of internodes. The total chlorophyll content of fully developed leaves wasmeasured by a UV–vis spectrophotometer at 645 and 663 nm following the method ofHiscox and Israelstam (1979)and results arepresented inTable 2. Occurrence of hyperhydricity was detectedby visible symptoms including thick, broad, translucent leaves thatwere wrinkled or curled, or both, and brittle (Table 3). Data onmicrotuberization was recorded after 10 weeks of culture; thenumber and fresh weight of microtubers was measured as an indexof their quality
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