Thai elite cassava (M. esculenta Crantz) cv. Rayong 90, Kasetsart 50 and Hauybong 60
and M. glaciovii wild species were used throughout. Field grown plant materials was brought in
vitro after scrub clean under running tap water and sterilization of shoot meristem and axillary
buds for 10 min in clorox 15%. Wash 2-3 times in steriled water. In vitro-grown of plants were
maintained on modified MS (Murashige and Skoog, 1962) inorganic medium containing 100
mg/l myo-inositol, 30 g/l sucrose and 8 g/l agar. Media were adjusted to pH 5.7 preior to
autoclaving at 121 0C for 25 min The plantlets were cultured at 25 0C under 8 h photoperiod (50