The essential oils and their volatile components may provide an important source of new anti-mycobacterial agents. Evaluations in checker-board assays of the individual components and their interactions of A. alata and S. glutinosa essential oils are necessary in order to determinate the active principles and toxicity of these complex mixes. Performing the macrodilution method with capped glass tubes is an alternative tool for obtaining more reproducible MIC values by controlling the essential oil high volatility.