The effect of basal media, BAP, Kn and sugars to the growth and development of Anoectochilus setaceus multiple shoot were reported in this study.
The basal ½ MS, MS and Knud medium were tested and shown to be equally suitable of them for shoot culture after 8 weeks.
The maintaining of culture until 12 weeks, growth of protocorms was superior in MS to that in ½ MS and Knud medium.
Other cultures were initiated from shoots inoculated onto MS medium supplemented individually with six different concentrations of 6-Benzylaminopurine (BAP) and Kinetin (Kn).
The highest number of shoots was obtained on medium supplemented with 0.6 mg l-1 BAP (4.4 ± 0.5 shoot/explant).
Out of all the investigated concentrations of Kn, the best result was obtained on medium supplemented with 1.0 mg l-1 Kn (3.3 ± 0.3 shoot/explant). In sugar study, results show that the shoot, root and leaf formation was significantly enhanced as the sugar concentration was decreased.
Medium supplemented with 2% (w/v) sucrose was the best compared to the other treatments and sugar at a concentration of 5% (w/v) induced the formation of large size seedlings.