About 50 mg of defatted rice flour, which was defatted with chloroform:methanol (2:1) for 16 h, was dispersed in 5 mL of 0.25 n KOH, and heated in a boiling water bath for 10 min. After cooling under a nitrogen purge, dispersion was neutralized with 0.1 n HCl and centrifuged at 5000 g for 10 min.