After incubation, the solutions should be immediately cooled in an ice-water bath and dissolved in deionized water (while the pH was adjusted to 4.8 with 1 M HCl) for 2 h at 25 C and then centrifuged at 10,000g for 30 min.
After incubation, the solutionsshould be immediately cooled in an ice-water bath and dissolvedin deionized water (while the pH was adjusted to 4.8 with 1 MHCl) for 2 h at 25 C and then centrifuged at 10,000g for 30 min.