Communities derived from TPW enrichments were characterized
by 454 pyrosequencing of the bacterial 16S rRNA gene
(Fig. 5), in order to provide a more in-depth view of the bacterial
communities present in selected samples. Amplified DNAs from
triplicate samples were pooled (as replicates had very similar
DGGE profiles [see Fig. S2A in the supplemental material]). Clus-
tering of quality-controlled sequences at the 95% similarity level,
and comparison of OTUs representing3% of the total community
were performed to identify the main changes in bacterial
community composition. All incubations were analyzed by 454
pyrosequencing, but the numbers of reads were low (100) in Pyr
samples and the no-added-PAH planktonic control, so they were
not included in the analysis.