Two tansy (Tanacetum vulgare L.) essential oils were obtained by steam distillation of the capitula with
subsequent liquid–liquid extraction (oil 1) or with use of an auxiliary phase for the trapping of the
steam components (oil 2). These oils were investigated against Bacillus subtilis F1276, B. subtilis spizizenii(DSM
618), Xanthomonas euvesicatoria, Pseudomonas syringae pv. maculicola, Ralstonia solanacearum
strain GMI1000 and Aliivibrio fischeri, using the coupling of high-performance thin-layer chromatography
to direct bioautography (HPTLC-DB).