The microbial sensitivity of the soil isolates
was analyzed by agar streak method. Each of
the isolate was streaked as a straight line on
SBCD medium and incubated at 27 °C for 6
days (144 h). After the 6th day, different
strains of microorganisms were streaked at
right angle, but not touching each other, and
then incubated at 37 °C for 24 h in the case
of bacteria and 27 °C for 48 h in the case of
fungi. If the organism is susceptible to the
antibiotic produced by actinomycetes, then it
will not grow near the actinomycetes. The
zone of inhibition against each test organism
was noted. The isolated actinomycetes were
screened against some microorganisms -
Bacillus subtilis, Staphylococcus aureus,
Escherichia coli, Pseudomonas aeruginosa,
Aspergillus niger and Candida albicans [8].
Based on their antimicrobial properties,
isolates were chosen for the further
biochemical characterization.